human il-6 (hil-6) (PeproTech)
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Human Il 6 (Hil 6), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il-6+(hil-6)/hil6/pmc12043090-195-5-10
Average 90 stars, based on 1 article reviews
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Sandwich ELISA:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. Enzyme-linked Immunosorbent Assay:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. Recombinant:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. Protease Inhibitor:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. Selection:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. Isolation:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. Cell Culture:Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs). Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant Article Title: Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer. Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines. Article Title: Lymphohematopoietic engineering using CAS9 base editors Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml Article Title: Use of tegaserod in preparation of anti-tumor drug Article Snippet: The recombinant Article Title: Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use. |
