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human il-6 (hil-6)  (PeproTech)


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    PeproTech human il-6 (hil-6)
    Human Il 6 (Hil 6), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il-6+%28hil-6%29/pmc12043090-195-5-10?v=PeproTech
    Average 90 stars, based on 1 article reviews
    human il-6 (hil-6) - by Bioz Stars, 2026-08
    90/100 stars

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    Effects <t>of</t> <t>IL-6</t> on cellular proliferation. (A) RNA and protein expression of IL-6 receptor in various cancer cells from the HPA database ( https://www.proteinatlas.org/ENSG00000160712-IL6R/cell+line , accessed on 29 Nov 2025). (B) Luciferase reporter assay for measuring RPMI 8226 cells activity with or without THP-1 cells, and IL-6 (5 ng/ml) for 48 hours. The seeding number of MM cells was 10,000. THP-1 cell numbers (THP-1 N.) were varied at 2000 (2K), 4000 (4K), 8000 (8K), and 10,000 (10K). (C) THP-1 cells were stimulated with IL-6 at concentrations of 0, 5, or 10 ng/ml for 24 hours. At the end of incubation, mRNA expression levels of iNOS and CD163 in THP-1 cells were quantified by qRT-PCR, and the ratio was determined by comparing the 2-ΔΔCt values of CD163 and iNOS. N = 9. ****p <0.0001.
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    R&D Systems hil 6 kit
    Effects <t>of</t> <t>IL-6</t> on cellular proliferation. (A) RNA and protein expression of IL-6 receptor in various cancer cells from the HPA database ( https://www.proteinatlas.org/ENSG00000160712-IL6R/cell+line , accessed on 29 Nov 2025). (B) Luciferase reporter assay for measuring RPMI 8226 cells activity with or without THP-1 cells, and IL-6 (5 ng/ml) for 48 hours. The seeding number of MM cells was 10,000. THP-1 cell numbers (THP-1 N.) were varied at 2000 (2K), 4000 (4K), 8000 (8K), and 10,000 (10K). (C) THP-1 cells were stimulated with IL-6 at concentrations of 0, 5, or 10 ng/ml for 24 hours. At the end of incubation, mRNA expression levels of iNOS and CD163 in THP-1 cells were quantified by qRT-PCR, and the ratio was determined by comparing the 2-ΔΔCt values of CD163 and iNOS. N = 9. ****p <0.0001.
    Hil 6 Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of IL-6 on cellular proliferation. (A) RNA and protein expression of IL-6 receptor in various cancer cells from the HPA database ( https://www.proteinatlas.org/ENSG00000160712-IL6R/cell+line , accessed on 29 Nov 2025). (B) Luciferase reporter assay for measuring RPMI 8226 cells activity with or without THP-1 cells, and IL-6 (5 ng/ml) for 48 hours. The seeding number of MM cells was 10,000. THP-1 cell numbers (THP-1 N.) were varied at 2000 (2K), 4000 (4K), 8000 (8K), and 10,000 (10K). (C) THP-1 cells were stimulated with IL-6 at concentrations of 0, 5, or 10 ng/ml for 24 hours. At the end of incubation, mRNA expression levels of iNOS and CD163 in THP-1 cells were quantified by qRT-PCR, and the ratio was determined by comparing the 2-ΔΔCt values of CD163 and iNOS. N = 9. ****p <0.0001.

    Journal: Frontiers in Immunology

    Article Title: Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapy

    doi: 10.3389/fimmu.2026.1760136

    Figure Lengend Snippet: Effects of IL-6 on cellular proliferation. (A) RNA and protein expression of IL-6 receptor in various cancer cells from the HPA database ( https://www.proteinatlas.org/ENSG00000160712-IL6R/cell+line , accessed on 29 Nov 2025). (B) Luciferase reporter assay for measuring RPMI 8226 cells activity with or without THP-1 cells, and IL-6 (5 ng/ml) for 48 hours. The seeding number of MM cells was 10,000. THP-1 cell numbers (THP-1 N.) were varied at 2000 (2K), 4000 (4K), 8000 (8K), and 10,000 (10K). (C) THP-1 cells were stimulated with IL-6 at concentrations of 0, 5, or 10 ng/ml for 24 hours. At the end of incubation, mRNA expression levels of iNOS and CD163 in THP-1 cells were quantified by qRT-PCR, and the ratio was determined by comparing the 2-ΔΔCt values of CD163 and iNOS. N = 9. ****p <0.0001.

    Article Snippet: Anti-human IL-6 was purchased from Invivogen (Cat #mabg-hil6-3).

    Techniques: Expressing, Luciferase, Reporter Assay, Activity Assay, Incubation, Quantitative RT-PCR

    Effects of metformin on RPMI 8226 cells with macrophages. (A) Luciferase reporter assay of RPMI 8226 cells following PBS control (Ctrl), metformin (Met. 1mM) or IL-6 (5ng/ml) treatment for 72 hours. (B) QRT-PCR analysis of CD163 and iNOS expression in THP-1 cells under various treatment conditions for 72 hours. (C) Flow cytometry analysis of CD126 expression on RPMI 8226 cells following 72 hours of metformin treatment with or without macrophages. (D) Luciferase reporter assay of RPMI 8226 cells under various treatment conditions at 72 hours. N = 4-9. *p<0.05, **p <0.01, ***p <0.001, ****p <0.0001.

    Journal: Frontiers in Immunology

    Article Title: Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapy

    doi: 10.3389/fimmu.2026.1760136

    Figure Lengend Snippet: Effects of metformin on RPMI 8226 cells with macrophages. (A) Luciferase reporter assay of RPMI 8226 cells following PBS control (Ctrl), metformin (Met. 1mM) or IL-6 (5ng/ml) treatment for 72 hours. (B) QRT-PCR analysis of CD163 and iNOS expression in THP-1 cells under various treatment conditions for 72 hours. (C) Flow cytometry analysis of CD126 expression on RPMI 8226 cells following 72 hours of metformin treatment with or without macrophages. (D) Luciferase reporter assay of RPMI 8226 cells under various treatment conditions at 72 hours. N = 4-9. *p<0.05, **p <0.01, ***p <0.001, ****p <0.0001.

    Article Snippet: Anti-human IL-6 was purchased from Invivogen (Cat #mabg-hil6-3).

    Techniques: Luciferase, Reporter Assay, Control, Quantitative RT-PCR, Expressing, Flow Cytometry