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human il-6 (hil-6)  (PeproTech)


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    Structured Review

    PeproTech human il-6 (hil-6)
    Human Il 6 (Hil 6), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il-6+(hil-6)/hil6/pmc12043090-195-5-10
    Average 90 stars, based on 1 article reviews
    human il-6 (hil-6) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sandwich ELISA:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.

    Enzyme-linked Immunosorbent Assay:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.

    Recombinant:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.

    Protease Inhibitor:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.

    Selection:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.

    Isolation:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.

    Cell Culture:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CD4-BV421 BD Biosciences Cat# 562424; RRID: AB_11154417 Anti-human CD4-PE BD Biosciences Cat# 555347; RRID: AB_395752 Anti-human CD3-PE BD Biosciences Cat# 566683; RRID: AB_2744380 Anti-human CD3-PE-Cy7 BD Biosciences Cat# 557749; RRID: AB_396855 Anti-human CD3-APC BD Biosciences Cat# 555342; RRID: AB_398592 Anti-human CD3-PerCP BD Biosciences Cat# 552851; RRID: AB_394492 Biological samples Human samples Shanghai Ninth People’s Hospital N/A Human samples Ruijin Hospital N/A Chemicals, peptides, and recombinant proteins Human IL-3 PEPROTECH Cat# 200-03 Human IL-6 PEPROTECH Cat# 200-06 Human SCF PEPROTECH Cat# 300-07 Human TPO PEPROTECH Cat# 300-18 Human IL-2 R&D Systems Cat# BT-002 Mouse IL-2 R&D Systems Cat# 402-ML APC-conjugated H-2Kb OVA Tetramer-SIINFEKL MBL Cat# TS-5001-2C Protease inhibitor cocktail Selleck Cat# B14001 Phosphatase inhibitor cocktail Selleck Cat# B15001 Sulfosuccinimidyl oleate MedChemExpress Cat# HY-112847 SC75741 MedChemExpress Cat# HY-10496 Fluvastatin MedChemExpress Cat# HY-14664 C75 MedChemExpress Cat# HY-12364 Bezafibrate MedChemExpress Cat# HY-B0637 Etomoxir MedChemExpress Cat# HY-50202 ABT-737 MedChemExpress Cat# HY-50907 Cytarabine MedChemExpress Cat# HY-13605 Coumermycin A1 MedChemExpress Cat# HY-N7452 Novobiocin MedChemExpress Cat# HY-B0425A TAK-242 MedChemExpress Cat# HY-11109 PP1 MedChemExpress Cat# HY-13804 Decitabine MedChemExpress Cat# HY-A0004 a-Tocopherol MedChemExpress Cat# HY-N0683 Busulfan MedChemExpress Cat# HY-B0245 Nor-NOHA ApexBio Technology Cat# C5407 OxLDL Solarbio Cat# IL2150 LDL Solarbio Cat# IL2110 HDL Solarbio Cat# IL2130 Palmitate Cayman Chemical Cat# 10006627 Oleic acid Cayman Chemical Cat# 90260 Arachidonic Acid Cayman Chemical Cat# 90010 CFSE Biolegend Cat# 423801 SIINFEKL peptide Nanjing Peptide Biotech N/A alkynyl palmitate click chemistry tools Cat# 1165 Critical commercial assays StemSpan SFEM II STEMCELL Technologies Cat# 09605_C EasySep Human Cord Blood CD34 Positive Selection Kit III STEMCELL Technologies Cat# 17897 (Continued on next page) e2 Cell Reports Medicine 5, 101592, June 18, 2024 Article ll OPEN ACCESS

    Article Title: Aging modulates the immunosuppressive, polarizing and metabolic functions of blood-derived myeloid-derived suppressor cells (MDSCs).
    Article Snippet: CD14 cells isolated either from PB or BCs were cultured with 5 × 105/ml in complete medium (10% FCS) in the presence of human IL-6 (20ng/ml) and GM-CSF (20ng/ ml) (Peprotech) for 5 days.

    Article Title: Butyrate Selectively Targets Super‐Enhancers and Transcriptional Networks Associated with Human Mast Cell Function
    Article Snippet: CD34 + cells were maintained for 4 weeks under serum‐free conditions using StemSpan medium (STEMCELL Technologies, 09650) supplemented with recombinant human IL‐6 (50 ng/mL; Peprotech, 200–06), human IL‐3 (10 ng/mL; Peprotech, 200–03), and human Stem Cell Factor (100 ng/mL Peprotech, 300–07).

    Article Title:
    Article Snippet: Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech, 900-M16).

    Article Title: Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer.
    Article Snippet: For single B cell sorting, B cells were enriched with anti-CD19 magnetic beads (Miltenyi Biotec, 130-050- 301) according to the manufacturer’s guidelines.

    Article Title: Lymphohematopoietic engineering using CAS9 base editors
    Article Snippet: Culturing media: StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies Catalog #09605); 100 ng/ml hSCF (Peprotech); 100 ng/ml hTPO (Peprotech); 100 ng/ml hFlt-3L (Peprotech); 100 ng/ml hIL-6 (Peprotech); StemRegenin1 (0.75 μM final concentration) Cayman Chemical Freezing Media: Cryostor CS10 Cell separation reagents: Human UCB CD34+ Enrichment Kit (Stem Cell Technologies; multiple variations are available depending on upon source).

    Article Title: Use of tegaserod in preparation of anti-tumor drug
    Article Snippet: The recombinant human IL-6 cytokine was purchased from Peprotech®.

    Article Title:
    Article Snippet: 51504) was used for extraction of DNA from fecal samples, the RNA later was removed from the patient's stool before Buffer ASL was added and the sample was then placed on a heat plate for heating at 70° C. for 5 minutes; next, added to the 0.1 mm sterilized microbeads (Model No.:BioSpec Products 0.1 MM ZIRCONIA/SILICA BEADS, Cat. No. 11079101z) and vortexed strongly for at least 15 seconds until the stool was mixed and crushed homogenously; the sample was then subjected to centrifugation at 13,000 rpm for 1 minute, the supernatant was collected for DNA extraction and the concentration of the extracted DNA was adjusted to 1 ng/μl for future use.



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    Effects of IL-6 on cellular proliferation. (A) RNA and protein expression of IL-6 receptor in various cancer cells from the HPA database ( https://www.proteinatlas.org/ENSG00000160712-IL6R/cell+line , accessed on 29 Nov 2025). (B) Luciferase reporter assay for measuring RPMI 8226 cells activity with or without THP-1 cells, and IL-6 (5 ng/ml) for 48 hours. The seeding number of MM cells was 10,000. THP-1 cell numbers (THP-1 N.) were varied at 2000 (2K), 4000 (4K), 8000 (8K), and 10,000 (10K). (C) THP-1 cells were stimulated with IL-6 at concentrations of 0, 5, or 10 ng/ml for 24 hours. At the end of incubation, mRNA expression levels of iNOS and CD163 in THP-1 cells were quantified by qRT-PCR, and the ratio was determined by comparing the 2-ΔΔCt values of CD163 and iNOS. N = 9. ****p <0.0001.

    Journal: Frontiers in Immunology

    Article Title: Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapy

    doi: 10.3389/fimmu.2026.1760136

    Figure Lengend Snippet: Effects of IL-6 on cellular proliferation. (A) RNA and protein expression of IL-6 receptor in various cancer cells from the HPA database ( https://www.proteinatlas.org/ENSG00000160712-IL6R/cell+line , accessed on 29 Nov 2025). (B) Luciferase reporter assay for measuring RPMI 8226 cells activity with or without THP-1 cells, and IL-6 (5 ng/ml) for 48 hours. The seeding number of MM cells was 10,000. THP-1 cell numbers (THP-1 N.) were varied at 2000 (2K), 4000 (4K), 8000 (8K), and 10,000 (10K). (C) THP-1 cells were stimulated with IL-6 at concentrations of 0, 5, or 10 ng/ml for 24 hours. At the end of incubation, mRNA expression levels of iNOS and CD163 in THP-1 cells were quantified by qRT-PCR, and the ratio was determined by comparing the 2-ΔΔCt values of CD163 and iNOS. N = 9. ****p <0.0001.

    Article Snippet: Anti-human IL-6 was purchased from Invivogen (Cat #mabg-hil6-3).

    Techniques: Expressing, Luciferase, Reporter Assay, Activity Assay, Incubation, Quantitative RT-PCR

    Effects of metformin on RPMI 8226 cells with macrophages. (A) Luciferase reporter assay of RPMI 8226 cells following PBS control (Ctrl), metformin (Met. 1mM) or IL-6 (5ng/ml) treatment for 72 hours. (B) QRT-PCR analysis of CD163 and iNOS expression in THP-1 cells under various treatment conditions for 72 hours. (C) Flow cytometry analysis of CD126 expression on RPMI 8226 cells following 72 hours of metformin treatment with or without macrophages. (D) Luciferase reporter assay of RPMI 8226 cells under various treatment conditions at 72 hours. N = 4-9. *p<0.05, **p <0.01, ***p <0.001, ****p <0.0001.

    Journal: Frontiers in Immunology

    Article Title: Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapy

    doi: 10.3389/fimmu.2026.1760136

    Figure Lengend Snippet: Effects of metformin on RPMI 8226 cells with macrophages. (A) Luciferase reporter assay of RPMI 8226 cells following PBS control (Ctrl), metformin (Met. 1mM) or IL-6 (5ng/ml) treatment for 72 hours. (B) QRT-PCR analysis of CD163 and iNOS expression in THP-1 cells under various treatment conditions for 72 hours. (C) Flow cytometry analysis of CD126 expression on RPMI 8226 cells following 72 hours of metformin treatment with or without macrophages. (D) Luciferase reporter assay of RPMI 8226 cells under various treatment conditions at 72 hours. N = 4-9. *p<0.05, **p <0.01, ***p <0.001, ****p <0.0001.

    Article Snippet: Anti-human IL-6 was purchased from Invivogen (Cat #mabg-hil6-3).

    Techniques: Luciferase, Reporter Assay, Control, Quantitative RT-PCR, Expressing, Flow Cytometry